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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes only the GAPDH protein
Cited Applications :WB
Cross Reactivity :Western blot GAPDH from Rabbit and Mouse
GAPDH from other species may also be detectable.
Immunogen :Synthetic peptide corresponding to a portion of the human G3PDH
Formulation :TBS, 50% glycerol
Purification :Affinity Chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Representative Western blot with Anti-GAPDH (1:1000) using 30μg of HeLa, 293, Jurkat and THP1 cell lysates.
Scientific Background :Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) catalyzes the sixth step of glycolysis – the oxidative phosphorylation of glyceraldehyde-3-phosphate – in the metabolic breakdown of glucose. GAPDH also plays an important role across a diverse array of biological functions such as endocytosis, mRNA regulation, and tRNA export, DNA replication, DNA repair, and apoptosis . GAPDH also serves as an ideal loading control or housekeeping gene .
1.Tarze, A, et al: GAPDH, a novel regulator of the pro-apoptotic mitochondrial membrane permeabilization. Oncogene. 2007. 26 (18): 2606-2620. PMID 17072346
2.Said, HM, et al: GAPDH is not regulated in human glioblastoma under hypoxic conditions. BMC Mol Biol. 2007 Jun 27; 8:55. PMID 17597534
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Research Areas :Metabolic Disorder, Apoptosis/Autophagy