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Description :Mouse Monoclonal Antibody
Specificity :Anti-GAPDH antibody recognizes the GAPDH protein.
Cited Applications :WB, ELISA
Cross Reactivity :GAPDH from Rabbit, Rat, Mouse, Human, Yeast, BL-21 Bacteria, and Sf9 insect cells
GAPDH from other species may also be detectable.
Host / Isotype / Clone# :Mouse, IgG1, GA1R
Immunogen :GAPDH from rabbit
Formulation :10mM PBS, pH 7.2
Purification :Purified by Protein A affinity chromatography from mouse ascites fluid
Stability :Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Representative western blot (1:1000) using 0.1µg, 0.5µg, and 1µg of HeLa cell lysate.
Scientific Background :Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) catalyzes the sixth step of glycolysis – the oxidative phosphorylation of glyceraldehyde-3-phosphate – in the metabolic breakdown of glucose. GAPDH also plays an important role across a diverse array of biological functions such as endocytosis, mRNA regulation, and tRNA export, DNA replication, DNA repair, and apoptosis . GAPDH also serves as an ideal loading control or housekeeping gene .
1. Tarze, A, et al: GAPDH, a novel regulator of the pro-apoptotic mitochondrial membrane permeabilization. Oncogene. 2007. 26 (18): 2606-2620. PMID 17072346.
2. Said, HM, et al: GAPDH is not regulated in human glioblastoma under hypoxic conditions. BMC Mol Biol. 2007 Jun 27;8:55. PMID 17597534.
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Research Areas :Metabolic Disorder, Apoptosis/Autophagy