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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the GAB1 protein
Cited Applications :WB
Cross Reactivity :Western blot human and mouse GAB1
GAB1 from other species may also be detectable.
Immunogen :Synthetic peptide corresponding to amino acids 625-629 of human GAB1
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Representative Western blot with Anti-GAB1 (1:1000) using 20ng of His-tagged human recombinant GAB1.
Sample Data :Representative Western blot with Anti-GAB1 (1:1000) using 3 μg, 10 μg and 30 μg of Jurkat cell lysate.
Scientific Background :GAB1 is a member of the IRS1-like multisubstrate docking protein family and is a direct substrate of the epidermal growth factor receptor and the insulin receptor (1). GAB1 is tyrosine phosphorylated upon stimulation of cells with various cytokines, growth factors, and antigen receptor. Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains such as SHP2 and phosphatidylinositol 3-kinase. GAB1 is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis (2).
1. Holgado-Madruga M, et al: A Grb2-associated docking protein in EGF- and insulin-receptor signalling. Nature 379: 560-564, 1996.
2. Kiyatkin A, et al: Scaffolding protein Grb2-associated binder 1 sustains epidermal growth factor-induced mitogenic and survival signaling by multiple positive feedback loops. J Biol Chem. 2006 Jul 21;281(29):19925-38.
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Research Areas :Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, ERK/MAPK Pathway, Invasion/Metastasis, JAK/STAT Pathway, Neurobiology, Cancer, Neurobiology, Cardiovascular Disease, ERK/MAPK Pathway, JAK/STAT Pathway, Apoptosis/Autophagy, Angiogenesis, Invasion/Metastasis