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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the FOXO1 protein
Cited Applications :WB
Cross Reactivity :Western blot of human and mouse FOXO1
FOXO1 from other species may also be detectable
Immunogen :Synthetic peptide corresponding to amino acids 317-321of human FOXO1
Formulation :PBS pH 7.4, 0.02% sodium azide, 50% glycerol
Purification :Affinity chromatography
Stability :Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Representative western blot with Anti-FOXO1 (1:1000) using 3 μg, 10 μg, and 30 μg of 3T6 cell lysate.
Scientific Background :Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21CIP1, a process negatively regulated through PI3K (1).
1. Seoane, J. et al: Integration of Smad and forkhead pathways in the control of neuroepithelial and glioblastoma cell proliferation. Cell. 2004 Apr 16;117(2):211-23.
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Research Areas :Cancer, Neurobiology, Cardiovascular Disease, Apoptosis/Autophagy, Cancer, Neurobiology, Cardiovascular Disease, Apoptosis/Autophagy