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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the EZH2 protein and is predicted to not cross-react with EZH1
Cited Applications :WB, ELISA, ICC, IF
Cross Reactivity :Human, Mouse and Rat
Host / Isotype / Clone# :Rabbit, IgG
Immunogen :EZH2 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human EZH2.
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Western blot analysis of EZH2 in 293 cell lysate with EZH2 antibody at 1 ug/mL.
Sample Data :Immunocytochemistry of EZH2 in 293 cells with EZH2 antibody at 10 ug/mL.
1. Chen H et al: Cloning of a human homolog of the Drosophila enhancer of zeste gene (EZH2) that maps to chromosome 21q22.2. Genomics 1996; 38:30-7
2. Margueron R et al: Ezh1 and Ezh2 maintain repressive chromatin through different mechanisms. Mol. Cell 2008; 32:503-18.
3. Shen X et al: EZH1 mediates methylation on histone H3 lysine 27 and complements EZH2 in maintaining stem cell identity and executing pluripotency. Mol. Cell 2008; 32:491-502.
4. Ezhkova E et al: EZH1 and EZH2 cogovern histone H3K27 trimethylation and are essential for hair follicle homeostasis and wound repair. Genes Dev. 2011; 25:485-98.
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Research Areas :Cancer, Cancer