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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the ELK1 protein
Cited Applications :WB
Cross Reactivity :Western blot human ELK1
ELK1 from other species may also be detectable.
Immunogen :Synthetic peptide corresponding to amino acids 387-391 of human ELK1
Purification :Affinity Chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Representative Western blot with Anti-ELK1 (1:1000) using 15ng of GST-tagged human recombinant ELK1.
Sample Data :Representative Western blot with Anti-ELK1 (1:1000) using 20μg of HeLa, 293, Jurkat and THP1cell lysates.
Scientific Background :ELK1 is a member of the ETS oncogene family of transcription factors (1). ELK1 protein interacts with promoter of the c-fos proto-oncogene and has been shown to be involved in the Ras-Raf-MAPK signaling cascade (1). Immature phosphorylation of transcription factor ELK-1 is implicated in premature aging syndrome and insulin resistance. Expression studies have revealed that elevated expression of ELK1-like protein in human esophageal can result in squamous cell carcinoma (2).
1.Chen, A.G. et al: Overexpression of Ets-like protein 1 in human esophageal squamous cell carcinoma. World J Gastroenterol. 2006, 28;12(48):7859-63.
2.Knebel, B. et al: Reduced phosphorylation of transcription factor Elk-1 in cultured fibroblasts of a patient with premature aging syndrome and insulin resistance. Exp Clin Endocrinol Diabetes. 2005;113(2):94-101.
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Research Areas :Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, ERK/MAPK Pathway, Invasion/Metastasis, Neurobiology, Cancer, Neurobiology, Cardiovascular Disease, ERK/MAPK Pathway, Apoptosis/Autophagy, Angiogenesis, Invasion/Metastasis