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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the DYKDDDDK tag
Cited Applications :WB
Cross Reactivity :All proteins tagged with DYKDDDDK epitope
Immunogen :DYKDDDDK peptide conjugated to KLH
Formulation :TBS, 50% glycerol
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Representative western blot with Anti-DYKDDDDK (1:5000) using 5 μg, and 15 μg of a positive cell lysate transfected with a DYKDDDDK tagged fusion protein.
Scientific Background :The DYKDDDDK octapeptide is small, highly charged, and hydrophilic, making it useful across a wide variety of applications . It is commonly employed in structural and functional studies where it is necessary to preserve the native confirmation and and/or activity of the protein .
1. Jarvik, JW. and Telmer, CA: Epitope tagging, Annual Review of Genetics, 1998; 32, 601-618.
2. Larson DM. et al: Functional expression and biochemical characterization of an epitope-tagged connexin37. Mol Cell Biol Res Commun 3: 2000; 115-121.
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