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  • $248.00


Description :Rabbit Polyclonal Antibody

Species :

Tag :

Expression System:

Sequence :

Specificity :Recognizes the DYKDDDDK tag

Cited Applications :WB

Cross Reactivity :All proteins tagged with DYKDDDDK epitope

Host :Rabbit

Immunogen :DYKDDDDK peptide conjugated to KLH

Purification :Affinity chromatography

Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data :Representative western blot with Anti-DYKDDDDK (1:5000) using 5 μg, and 15 μg of a positive cell lysate transfected with a DYKDDDDK tagged fusion protein.

Scientific Background :The DYKDDDDK octapeptide is small, highly charged, and hydrophilic, making it useful across a wide variety of applications [1]. It is commonly employed in structural and functional studies where it is necessary to preserve the native confirmation and and/or activity of the protein [2].

References :
1. Jarvik, JW. and Telmer, CA: Epitope tagging, Annual Review of Genetics, 1998; 32, 601-618.

2. Larson DM. et al: Functional expression and biochemical characterization of an epitope-tagged connexin37. Mol Cell Biol Res Commun 3: 2000; 115-121.

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