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Anti-DOK1(D42-63R)

Anti-DOK1(D42-63R)

  • $270.00


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Description :Rabbit Polyclonal Antibody

Species :

Tag :

Expression System:

Sequence :

Specificity :Recognizes the DOK1 protein

Cited Applications :WB

Cross Reactivity :Western blot DOK1 from human and mouse cells

DOK1 from other species may also be detectable.

Host :Rabbit

Immunogen :Synthetic peptide corresponding to amino acids 360-364 of human DOK1 (Q99704)

Purification :Affinity chromatography

Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data :Representative western blot with Anti-DOK1 (1:1000) using 3 μg, 10 μg and 30 μg of 3T6 cell lysate.

Scientific Background :DOK1 contains pleckstrin homology domain and ten PXXP SH3 recognition motifs and is a critical substrate for p210 bcr/abl. DOK1 is constitutively tyrosine phosphorylated in hematopoietic progenitors cells isolated from CML patients (1). DOK1 is associated with p120 Ras-GAP and this association is correlated with its tyrosine phosphorylation. Aberrant phosphorylation of DOK1 may contribute to the progression of different human diseases (2).

References :
1. Carpino, N. et.al: p62(dok): a constitutively tyrosine-phosphorylated, GAP-associated protein in chronic myelogenous leukemia progenitor cells. Cell 88: 197-204, 1997.

2. Yasuda. et.al: Role of Dok-1 and Dok-2 in myeloid homeostasis and suppression of leukemia. J. Exp. Med. 200: 1681-1687, 2004.

Product Sheets (By Lot #) :

R039-7.pdf

Research Areas :Cancer, ERK/MAPK Pathway, Cancer, ERK/MAPK Pathway