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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the DNMT3L protein
Cited Applications :ELISA, IP, WB
Cross Reactivity :Human
Host Isotype / Clone# :Rabbit, IgG
Immunogen :The antibody was produced against synthesized peptide corresponding to amino acids 152-164 of human DNMT3L (DNA (cytosine-5-)-methyltransferase-3-like).
Formulation :0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 + 0.01% (w/v) Sodium Azide
Purification :Immunoaffinity chromatography
Stability :1yr at –20oC from date of shipment
Sample Data :Immunohistochemistry Rabbit Anti-DNMT3L 1:500
Sample Data :Western blot using affinity purified Anti-DNMT3L Antibody (1:1,500) after immunoprecipitation. Transfection of U2OS cells (10cm dish) was accomplished using 3 ?g of GAL4-DNMT3L.
Scientific Background :DNMT3L is an enzymatically inactive regulatory factor that shares sequence homology with the DNA methylatransferase DNMT3A and DNMT3B. DNMT3L is involved in the establishment of DNA methylation patterns during gametogenesis. In mammals, DNA methylation is used for heritable silencing of retrotransposons and imprinted genes and for inactivation of the X chromosome in females (1). DNMT3L has a genomewide role in CpG island methylation beyond genomic imprinting (2). DNMT3L functions as a regulators of methylation at imprinted loci rather than a DNA cytosine methyltransferase because of a lack of catalytic motifs in its sequence.
1. Ooi, S.K.T. et al: DNMT3L connects unmethylated lysine 4 of histone H3 to de novo methylation of DNA. Nature 448: 714-717, 2007.
2. Smallwood, S. A. et al: Dynamic CpG island methylation landscape in oocytes and preimplantation embryos. Nature Genet. 43: 811-814, 2011.
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Research Areas :Apoptosis/Autophagy, Cancer, Neurobiology