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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the DLK1 protein
Cited Applications :WB, ELISA
Cross Reactivity :Human, Mouse and Rat
Host / Isotype / Clone# :Rabbit, IgG
Immunogen :DLK1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human DLK1
Formulation :PBS + 0.02% sodium azide.
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Western blot analysis of HepG2 in 293 cell lysate with DLK1 antibody at (A) 1 and (B) 2 ug/ml.
Scientific Background :DLK or mitogen-activated protein kinase kinase kinase 12 is a transmembrane protein containing six epidermal growth factor repeats and is a serine/threonine protein kinase. DLK is involved in the differentiation of several cell types, including adipocytes. DLK plays a role of a tumor suppressor protein and is predominately expressed in neuronal cells. DLK as an activator of the Jnk pathway. Overexpression of DLK results in activation of Jnk1 and the accumulation of a hyperphosphorylated form of c-Jun (1). Postnatal loss of DLK in stem cells and niche astrocytes has been shown to regulate neurogenesis (2).
1. Hirai, S. et al: Activation of the JNK pathway by distantly related protein kinases, MEKK and MUK. Oncogene 12: 641-650, 1996.
2. Ferron, S. R.et.al: Postnatal loss of Dlk1 imprinting in stem cells and niche astrocytes regulates neurogenesis. Nature 475: 381-385, 2011.
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Research Areas :Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, ERK/MAPK Pathway, Invasion/Metastasis, Neurobiology, Ser/Thr Kinases, Cancer, Neurobiology, Cardiovascular Disease, ERK/MAPK Pathway, Apoptosis/Autophagy, Angiogenesis, Invasion/Metastasis, Ser/Thr Kinases