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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the DAPK3 protein
Cited Applications :WB, ELISA, ICC, IF
Cross Reactivity :Human, Mouse and Rat
Host / Isotype / Clone# :Rabbit, IgG
Immunogen :DAPK3 antibody was raised against a peptide corresponding to amino acids near the center of human DAPK3.
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Western blot analysis of DAPK3 in HeLa (H) and Jurkat (J) whole cell lysates with DAPK antibody at 1 ug/ml.
Sample Data :Immunocytochemistry of DAPK3 in Jurkat cells with DAPK3 antibody at 10 ug/ml.
Scientific Background :DAPK3 or Death-associated protein kinase 3 (also known as ZIP) plays a role in apoptosis (1). DAPK3 is a nuclear serine/threonine-specific kinase that phosphorylates core histones H3 and H4, and myosin light chain in vitro. DAPK3 interacts with transcription and splicing factors as well as with pro-apoptotic protein Par-4 suggesting that it participates in multiple cellular processes. DAPK3 contains a leucine zipper structure at its C terminus and this region is responsible for binding to ATF4. The leucine zipper domain is necessary for the homodimerization of DAPK3 as well as for the activation of the kinase (2).
1. Kawai, T. et al: ZIP kinase, a novel serine/threonine kinase which mediates apoptosis. Mol. Cell Biol. 1998;18(3):1642-51.
2. Preuss, U. et al. Novel mitosis-specific phosphorylation of histone H3 at Thr11 mediated by Dlk/ZIP kinase. Nucleic Acids Res. 2003; 31(3):878-85.
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Research Areas :Apoptosis/Autophagy, Cancer, Cellular Stress, Inflammation, Neurobiology, Ser/Thr Kinases, Cancer, Neurobiology, Inflammation, Apoptosis/Autophagy, Cellular Stress