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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the DAPK2 protein and has no cross response with DAPK1
Cited Applications :WB, ELISA, IHC
Cross Reactivity :Human, Mouse and Rat
Host / Isotype / Clone# :Rabbit, IgG
Immunogen :DAPK2 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human DAPK2. This antigenic peptide is identical to the corresponding amino acids of mouse origin.
Formulation :PBS + 0.02% sodium azide.
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Western blot analysis of DAPK2 in A431 (H), mouse spleen (M), and rat kidney (R) lysates with DAPK2 antibody at 1 ug/ml.
Sample Data :Immunohistochemistry of DAPK2 in mouse spleen cells with DAPK2 antibody at 2 ug/ml.
Scientific Background :DAPK2 or death-associated protein kinase 2 belongs to a family of proapoptotic Ca(2+)/calmodulin-regulated serine/threonine kinases. Overexpression of DAPK2 induces cell apoptosis. DAPK2 has been shown to be a novel Sp1-dependent target gene for E2F1 and Krüppel-like factor 6 (KLF6) in cell death response (1). Both E2F1 and KLF6 strongly activate the DAPK2 promoter. DAPK2 plays a role in granulopoiesis where it is highly expressed. β-catenin can block anoikis of malignant kidney and intestinal epithelial cells and promote their anchorage-independent growth by down-regulating DAPK2 (2). β-catenin-induced down-regulation of DAPK2 requires the presence of the transcription factor TCF-4.
1. Britschgi A. et al: DAPK2 is a novel E2F1/KLF6 target gene involved in their proapoptotic function. Oncogene. 2008 Sep 25;27(43):5706-16.
2. Li, H. et al: Down-regulation of death-associated protein kinase-2 is required for beta-catenin-induced anoikis resistance of malignant epithelial cells. J Biol Chem. 2009 Jan 23;284(4):2012-22.
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Research Areas :Apoptosis/Autophagy, Cancer, Cellular Stress, Inflammation, Neurobiology, Ser/Thr Kinases, Cancer, Neurobiology, Inflammation, Apoptosis/Autophagy, Cellular Stress