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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the human Cyclin E2 protein
Cited Applications :ELISA, IP, WB
Ideal working dilutions for each application should be empirically determined by the investigator.
Cross Reactivity :Human and Mouse
Host Isotype / Clone# :Rabbit, IgG
Immunogen :The antibody was produced against synthesized peptide corresponding to amino acids at the carboxyl terminus of Cyclin E2 protein
Formulation :0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 + 0.01% (w/v) Sodium Azide
Purification :Immunoaffinity chromatography
Stability :1yr at –20oC from date of shipment
Sample Data :Western blot using affinity purified Anti-Cyclin E2 antibody shows specific detection of Cyclin E2. Cell extracts over-expressing mouse Cyclin E1 (lane 1) and Cyclin E2 (lane 2) were electrophoresed, transferred to nitrocellulose, and probed with the anti-Cyclin E2 antibody.
Scientific Background :Cyclin E was first identified by its ability to rescue growth of yeast deficient in G1 Cyclins, indicating a role in G1 or G1/S transitions. Over-expression of Cyclin E has been observed in a variety of human tumors. Multiple isoforms of Cyclin E are expressed in tumors but not in normal tissues, suggesting a post-transcriptional regulation of Cyclin E. Cyclin E2 associates with Cdk2 in a functional kinase complex that is inhibited by both p27Kip1 and p21Cip1. The catalytic activity associated with Cyclin E2 complexes is cell cycle regulated and peaks at the G1/S transition. Unlike Cyclin E1, which is expressed in most proliferating normal and tumor cells, Cyclin E2 levels were low to undetectable in non-transformed cells and increased significantly in tumor-derived cells.
1. Philipp, G.Y., et al.:Cyclin E ablation in the mouse. Cell 114(4):431-443. Yu, Q., Sicinski, P. (2004) Mammalian cell cycles without Cyclin E-CDK2. Cell Cycle 2003: 3(3); 292-295.
2. Parisi, T., et al.: Cyclins E1 and E2 are required for endoreplication in placental trophoblast giant cells. EMBO J. 2003: 22(18); 4794-4803.
3. Geng, Y., et al.: Expression of Cyclins E1 and E2 during mouse development and in neoplasia. Proc. Natl. Acad. Sci. USA 2001: 98(23); 13138-13143.
4. Berthet, C., et al.: Cdc2-Cyclin E complexes regulate the G1/S phase transition. Nat. Cell Biol. 2005: 7(8); 831-836.
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Research Areas :Cancer, Cell Cycle, Ser/Thr Kinases