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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the 45kDa Cyclin E protein
Cited Applications :ELISA, IP, WB
Ideal working dilutions for each application should be empirically determined by the investigator.
Cross Reactivity :Human
Host Isotype / Clone# :Rabbit, Antiserum
Immunogen :The antibody was produced against synthesized peptide corresponding to a region near the carboxy terminus of human cyclin E conjugated to KLH.
Formulation :0.01% (w/v) Sodium Azide
Purification :Delipidation and defibrination
Stability :1yr at –20oC from date of shipment
Scientific Background :Anti-Cyclin-E antibody detects cyclin-E. Cyclin E is a member of the cyclin family. Cyclin E binds to the G1 phase Cdk2, which is required for the transition from G1 to S phase of the cell cycle that determines cell division. The Cyclin E/CDK2 complex phosphorylates p27Kip1, tagging it for degradation, thus promoting expression of Cyclin A, allowing progression to S phase. Anti-cyclin-e Antibody is ideal for investigators involved in Cell Signaling, cell biology and Signal Transduction research.
1. Koff, A., et al.: Human cyclin E, a new cyclin that interacts with two members of the CDC2 gene family. Cell. 1991: 66; 1217-1228.
2. Koff, A., et al.: Formation and activation of a cyclin E-cdk2 complex during the G1 phase of the human cell cycle. Science. 1992: 257; 1689-1694.
3. Claudio, P.P., et al.: Functional analysis of pRb/p130 intereaction with cyclins. Cancer Res. 1996: 56; 2003-2008.
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Research Areas :Cancer, Cell Cycle, Ser/Thr Kinases