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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the 55-66kDa Cyclin B1 protein
Cited Applications :ELISA, IP, WB
Ideal working dilutions for each application should be empirically determined by the investigator.
Cross Reactivity :Human, Mouse and Rat
Host Isotype / Clone# :Rabbit, Antiserum
Immunogen :The antibody was produced against full length fusion protein corresponding to the human Cyclin B1 protein.
Purification :Delipidation and Defibrination
Stability :1yr at –20oC from date of shipment
Sample Data :Western blot analysis using Anti-Cylcin B1 antibody (1:500) shows detection of ~62kDa human Cyclin B1 present in asynchronous HN30 cell lysates. HN30 cells are from head and neck cancer tumors that over express cyclin B1 and D1.
Scientific Background :The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product forms a complex with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.
1. Giordano, A., et al.: A 60 kd cdc-2 associated polypeptide complexes with the E1A proteins of adenovirus-infected cells. Cell. 1989: 58; 981-990.
2. Pines, J., et al.: Human cyclin A is adenovirus E1A-associated protein p60 and behaves differently from cyclin B. Nature. 1990: 346; 760-763.
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Research Areas :Cancer, Cell Cycle, Ser/Thr Kinases