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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the Cyclin A protein
Cited Applications :WB, ELISA, IP
Cross Reactivity :Human, Mouse and Rat
Host / Isotype / Clone# :Rabbit, IgG
Immunogen :Anti-Cyclin A Antibody was produced by repeated immunizations with a recombinant protein corresponding to the human cyclin A.
Formulation :0.01% (w/v) Sodium Azide
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Immunohistochemistry using Rabbit Anti-Cyclin A antibody. Tissue:human testes tissue. Fixation: formalin fixed paraffin embedded.Antigen retrieval: not required.Primary antibody: Anti-Cyclin A at 1:500 for 1 hr at RT. Localization: Cyclin A is nuclear and cytoplasmic.Staining: Cyclin A as precipitated red signal with hematoxylin purple nuclear counterstain.
Sample Data :Western Blot of Rabbit Anti-Cyclin A antibody. Lane 1: T98G cells treated with normal (n). Lane 2: T98G cells treated with scrambled (scr).Lane 3: T98G cells treated with PIN1 knockdown (kd1).Load: 25 µg per lane. Primary antibody: Anti-Cyclin A at 1:400 for overnight at 4°C. Predicted/Observed size: 60kDa for Cyclin A.
Scientific Background :Anti-Cyclin A detects cyclin a. Cyclin A is a member of the cyclin family. Cyclin A binds to S phase CDK2 and is required for the cell to progress through the S phase. CDK2/Cyclin A is inhibited by the complex p21CIP. The Anti-Cyclin-A antibody is ideal for investigators involved in cell signaling, cell biology and signal transduction research.
References :1. Rizzolio F, Lucchetti C, et al, Retinoblastoma tumor-suppressor protein phosphorylation and inactivation depend on direct interaction with Pin1. Cell Death and Differentiation 1-10. Nature 2012.
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Research Areas :Cancer, Cell Cycle, Cancer, Cell Cycle