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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the CREB protein
Cited Applications :WB, IP
Cross Reactivity :Western blot and immunoprecipitation of human and mouse CREB
CREB from other species may also be detectable.
Immunogen :Synthetic peptide corresponding to amino acids 699-703 of human STAT1
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Representative immunoprecipitation with 2 μg of Anti-CREB using 500 ug of HeLa, HEK-293T, Jurkat, and 3T6 cell lysate.
Sample Data :Representative western blot with Anti-CREB (1:1000) using 30 ug of HeLa, HEK-293T, Jurkat, THP1, and 3T6 cell lysate.
Scientific Background :CREB1 is a member of the cAMP responsive element binding protein family of transcription factors that are critical mediators of gene expression in response to extracellular signals and are essential regulators of adaptive behavior and long-term memory formation (1). Activation of CREB1 by protein kinase A-mediated phosphorylation has been implicated in the survival of mammalian cells. Mice lacking CREB in the central nervous system during development show extensive apoptosis of postmitotic neurons (2). CREB binds as homo- and heterodimers to promoters containing CRE and activator protein 1 (AP-1) sites to alter target-gene expression).
1. Valverde, O. et al: Modulation of anxiety-like behavior and morphine dependence in CREB-deficient mice. Neuropsychopharmacology. 2004 Jun; 29(6): 1122-33.
2. Mantamadiotis, T. et al: Disruption of CREB function in brain leads to neurodegeneration. Nat Genet. 2002 May; 31(1): 47-54.
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Research Areas :Neurobiology, Cardiovascular Disease, ERK/MAPK Pathway, NfkB Pathway, PKA/PKC Pathway, Apoptosis/Autophagy, Neurobiology, Cardiovascular Disease, ERK/MAPK Pathway, NfkB Pathway, PKA/PKC Pathway, Apoptosis/Autophagy