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Description :Goat Polyclonal Antibody
Specificity :Recognizes the choline acetyltransferase protein
Cited Applications :WB, IHC
Cross Reactivity :Human, Mouse, Rat, Chicken, Guinea Pig and Non-Human Primates
Host / Isotype / Clone# :Goat, IgG
Immunogen :Native choline acetyltransferase purified from human placenta
Formulation :100 ul in PBS + 5 mg/ml BSA + 0.2% NaN3
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Western blot of rat brain lysate showing specific immunolabeling of the ~70k Choline Acetyltransferase.
Scientific Background :Choline acetyltransferase (ChAT )is a neuronal enzyme which catalyzes the reaction between Acetyl CoA and choline resulting in the formation of acetylcholine. It is therefore found primarily in cholinergic neurons making it a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease (AD) and Down syndrome (2). Decreased choline acetyltransferase activity in particular has been shown in Schizophrenic subjects (1). It has furthermore been demonstrated that in patients with AD, there are significantly lower levels of cortical ChAT that correlate with severity of the disease as measured by loss of neuropsychological function (3).
1. Karson CN, Casanova MF, Kleinman JE, Griffin WS Choline acetyltransferase in schizophrenia. Am. J.Psychiatry 150:454-459. 1993.
2. Holt DJ, et al Evidence for a deficit in cholinergic interneurons in the striatum in schizophrenia. Neuroscience. 94(1):21-31. 1999.
3. Baskin DS, et al, Brain choline acetyltransferase and mental function in Alzheimer disease. Arch Neurol. 56:1121-1123.1999.
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Research Areas :Neurobiology