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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the CHK2 protein
Cited Applications :WB, ELISA, ICC
Cross Reactivity :Human, Mouse and Rat
Host / Isotype / Clone# :Rabbit, IgG
Immunogen :CHK2 antibody was raised against a synthetic peptide corresponding to amino acids near the amino terminus of human CHK2
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Western blot analysis of CHK2 expression in K562 (A), Jurkat (B), and HL-60 (C) whole cell lysates with CHK2 antibody at 1 ug /ml.
Sample Data :Immunocytochemistry of CHK2 in Jurkat cells with CHK2 antibody at 1 ug/ml.
Scientific Background :CHK2 is rapidly phosphorylated and activated in response to replication blocks and DNA damage; the response to DNA damage occurs in an ataxia telangiectasia mutated (ATM)-dependent manner (1). Expression of wild-type Chk2 leads to increased p53 stabilization after DNA damage, whereas expression of a dominant-negative Chk2 mutant abrogated both phosphorylation of p53 on Ser-20 and p53 stabilization (2).
1. Matsuoka, S. et al: Linkage of ATM to cell cycle regulation by the Chk2 protein kinase. Science. 1998 Dec 4; 282(5395):1893-7.
2. Chehab NH. et al: Chk2/hCds1 functions as a DNA damage checkpoint in G(1) by stabilizing p53. Genes Dev. 2000 Feb 1; 14(3):278-88.
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Research Areas :Cancer, Cell Cycle, Ser/Thr Kinases, Cell Cycle, Ser/Thr Kinases