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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the 35kDa CDK5 (p31) protein
Cited Applications :ELISA, IP, WB
Ideal working dilutions for each application should be empirically determined by the investigator.
Cross Reactivity :Human, Rat and Mouse
Host Isotype / Clone# :Rabbit, Antiserum
Immunogen :The antibody was produced against synthesized peptide corresponding to the C-Terminus of human CDK5 protein conjugated to KLH.
Purification :Delipidation and defibrination
Stability :1yr at –20oC from date of shipment
Sample Data :Anti-CDK5 antibody was diluted 1:500 to detect CDK5 in human brain cortex tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
Scientific Background :The neuronal protein kinase, CDK5 is implicated in a vast array of normal neuronal functions including regulation of neurotransmitter synthesis (1), the presynaptic vesicle cycle (2), neurotransmitter receptor trafficking, and dopamine neurotransmission (3). CDK5 is active as a heterodimer with a 25 kDa protein. The 25 kDa protein is derived proteolytically from a 35 kDa brain and neuron-specific precursor protein (4). CDK5 phosphorylates a diverse list of substrates and has been implicated in a wide range of neurological and neuropsychiatric disorders, which include Alzheimer’s, Parkinson’s, Huntington’s, epilepsy, schizophrenia, and drug addiction (5).
1. Kansy, J. et al. Identification of tyrosine hydroxylase as a physiological substrate of Cdk5. J. Neurochem. 2004 91, 374-384.
2. Nguyen, C. et al: Cdk5 and the mystery of synaptic vesicle endocytosis. J Cell Biol. 2003 163, 697- 699. Bibb, J A. et al. Phosphorylation of DARPP-32 by Cdk5 modulates dopamine signaling in neurons. Nature 1999 402, 669-671.
3. Tang, D. et al: Cyclin-dependent kinase 5 (Cdk5) and neuron-specific Cdk5 activators. Prog Cell Cycle Res. 1996;2:205-16.
4. Dhavan, R. et al: A decade of CDK5. Nat Rev Mol Cell Biol. 2001 Oct;2(10):749-59.
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Research Areas :Cancer, Cell Cycle, Ser/Thr Kinases