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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the 24kDa CDK4 (p34) protein.
Cited Applications :ELISA, IP, WB
Ideal working dilutions for each application should be empirically determined by the investigator.
Cross Reactivity :Human, Rat and Mouse
Host Isotype / Clone# :Rabbit, Antiserum
Immunogen :The antibody was produced against synthesized peptide corresponding to the C-Terminus of the human CDK4 (p34) protein conjugated to KLH.
Formulation :0.01% (w/v) Sodium Azide
Purification :Delipidation and Defibrination
Stability :1yr at –20oC from date of shipment
Scientific Background :CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase of cell cycle. CDK4 forms a complex with the D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). CDK4 can mediate phosphorylation of the C-terminal region of RB protein leading to an active transcriptional repression of E2F complex (1). CDC37 and HSP90 can preferentially associate with the fraction of CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2 (2).
1. Harbour, J W. et al: Cdk phosphorylation triggers sequential intramolecular interactions that progressively block Rb functions as cells move through G1. Cell 98: 859-869, 1999.
2. Matsuura, I. et al: Cyclin-dependent kinases regulate the antiproliferative function of Smads. Nature 430: 226-231, 2004.
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Research Areas :Cancer, Cell Cycle, Ser/Thr Kinases