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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the CDC25A protein
Cited Applications :WB
Cross Reactivity :Western blot human and mouse CDC25A
CDC25A from other species may also be detectable.
Immunogen :Synthetic peptide corresponding to amino acids 74-78 of human CDC25A
Formulation :PBS pH 7.4, 0.02% NaN3, 50% glycerol
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Representative Western blot with Anti-CDC25A (1:1000) using 3 μg, 10 μg and 30 μg of Hela cell lysate.
Scientific Background :CDC25A is a member of the CDC25 family of phosphatases and is required for cell cycle progression from G1 to S phase. CDC25A activates the cyclin-dependent kinases by removing two inhibitory phosphate groups on the CDKs. CDC25A is a potential oncogene since it promotes cell division. CDC25A is over-expressed in a number of cancers and functions as an androgen receptor (AR) co-regulator suppressing the AR transcriptional activity. Human cells respond to ultraviolet light or ionizing radiation by rapid, ubiquitin- and proteasome-dependent protein degradation of CDC25A (1).
1. Falck, J. et al: The ATM-Chk2-Cdc25A checkpoint pathway guards against radioresistant DNA synthesis. Nature 410842-847, 2001.
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Research Areas :Cancer, ERK/MAPK Pathway, Inflammation, Invasion/Metastasis, Neurobiology, Cancer, Neurobiology, Inflammation, ERK/MAPK Pathway, Invasion/Metastasis