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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the human CaM Kinase IV protein
Cited Applications :ELISA, WB
Cross Reactivity :Human, Mouse, Rat, Dog, Bovine, Chimpanzee
Host Isotype / Clone# :Rabbit, Antiserum
Immunogen :The antibody was produced against synthesized peptide corresponding to amino acids 305-323 of Human CaM Kinase IV protein.
Formulation :0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 + 0.01% (w/v) Sodium Azide
Purification :Delipidation, defibrination, buffering and clarification.
Stability :1yr at –20oC from date of shipment
Sample Data :Western blot using Anti-CAMK4 (1:1,000) shows detection of a band ~52 kDa corresponding to CaM Kinase IV (arrowhead) in various preparations: lane 1 - rat brain lysate, lane 2 - Jurkat cell lysate. Specific reactivity is blocked in both lysates when antibody is preincubated with immunizing peptide (lanes 3 and 4 respectively). Approximately 35 µg of each lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. CaM Kinase IV was similarly detected on lysates from mouse brain (not shown).
Sample Data :Anti-CAMK4 antibody was diluted 1:500 to detect CAMK4 in human brain cortex tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
Scientific Background :CAMK4 is a multifunctional serine/threonine protein kinase and a member of Ca(2+)/calmodulin- dependent protein kinase family. CAMK4 is localized in neurons in the hippocampus, amygdala, anterior cingulate cortex, somatosensory cortex, and insular cortex (1). CAMK4 is involved in neural activity-dependent signaling in the neuronal nucleus and thought to plays an important role in the consolidation/retention of hippocampus-dependent long-term memory (2).
1. Sikela, J. M. et al: Chromosomal localization of the human gene for brain Ca(2+)/calmodulin-dependent protein kinase type IV. Genomics 4: 21-27, 1989.
2. Kang, H. et al: An important role of neural activity-dependent CaMKIV signaling in the consolidation of long-term memory. Cell 106: 771-783, 2001.
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Research Areas :Cardiovascular Disease, ERK/MAPK Pathway, Neurobiology, PKA/PKC Pathway, Ser/Thr Kinases