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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the human CaM Kinase II Protein
Cited Applications :ELISA, WB
Ideal working dilutions for each application should be empirically determined by the investigator.
Cross Reactivity :Human, Mouse, Rat, Dog and Orangutan
Host Isotype / Clone# :Rabbit, IgG
Immunogen :The antibody was produced against synthesized peptide corresponding to amino acids 6-23 of Human CaM Kinase II protein.
Formulation :0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 + 0.01% (w/v) Sodium Azide
Purification :Affinity chromatography
Stability :1yr at –20oC from date of shipment
Sample Data :Western blot using Anti-CAMK2 (1:1,500) shows detection of a band ~54 kDa corresponding to human alpha CaM Kinase II Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (lane 2).
Scientific Background :CaM Kinase II (also known as CAMK2 and calcium/calmodulin-dependent protein kinase type II alpha chain) is a prominent kinase in the central nervous system that may function in the long-term potentiation of neurotransmitter release. CaM Kinase II autophosphorylates itself at Thr-286 which allows the kinase to switch from a calmodulin-dependent to a calmodulin-independent state. CaM Kinase II is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. This kinase is expressed in brain tissue. Alternative splicing occurs for this gene product.
1. Lin,C.R., et al.: Molecular cloning of a brain-specific calcium/calmodulin-dependent protein kinase. Proc. Natl. Acad. Sci. U.S.A. 1987: 84 (16); 5962-5966.
2. Bulleit,R.F., et al.: Conserved and variable regions in the subunits of brain type II Ca2+/calmodulin-dependent protein kinase. Neuron 1. 1988: (1); 63-72. Hanley,R.M., et al.: Functional analysis of a complementary DNA for the 50-kilodalton subunit of calmodulin kinase II. Science.1987: 237 (4812); 293-297.
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Research Areas :Cardiovascular Disease, ERK/MAPK Pathway, Neurobiology, PKA/PKC Pathway, Ser/Thr Kinases