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Description :Mouse Monoclonal Antibody
Specificity :Recognizes the CAMK1 protein
Cited Applications :WB
Cross Reactivity :Western blot human CAMK1
CAMK1 from other species may also be detectable.
Host / Isotype / Clone# :Mouse, IgG1, 5H8
Immunogen :Human recombinant full length CAMK1 protein
Formulation :0.1M TRIS pH 8.0, 0.1M glycine
Purification :Affinity purified using Protein G
Stability :Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :
Representative western blot (1:1000) using 20ng of CAMK1, CAMK1β, CAMK1γ, CAMK1δ, CAMK2α, CAMK2β, CAMK2γ, and CAMK4 recombinant protein.
Scientific Background :CAMK1 is a serine/threonine protein kinase that is a member of the multifunctional calcium/calmodulin-dependent protein kinase family. CAMK1 is ubiquitously expressed and phosphorylates a number of proteins including SYN1, SYN2, CREB and CFTR. In addition, Numb family of proteins may also be intracellular targets for CAMK1, and they may also be regulated by phosphorylation-dependent interaction with 14-3-3 protein (1). CAMK1 also plays an important role in the trafficking of HDAC7 between the cytoplasm and the nucleus. CAMK1 phosphorylates HDAC7 on multiple sites that lead to alteration in localization of HDAC7 (2).
References :1. Tokumitsu, H. et al: Phosphorylation of Numb family proteins. Possible involvement of Ca2+/calmodulin-dependent protein kinases. J Biol Chem. 2005 Oct 21;280(42):35108-18.
2. Gao, C. et al: CRM1 mediates nuclear export of HDAC7 independently of HDAC7 phosphorylation and association with 14-3-3s. FEBS Lett. 2006 Sep 18;580(21):5096-104.
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Research Areas :Neurobiology, Cardiovascular Disease, ERK/MAPK Pathway, PKA/PKC Pathway, Neurobiology, Cardiovascular Disease, ERK/MAPK Pathway, PKA/PKC Pathway