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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the BTK protein
Cited Applications :WB, ELISA, ICC, IF
Cross Reactivity :Human and Mouse
Host / Isotype / Clone# :Rabbit, IgG
Immunogen :BTK antibody was raised against a synthetic peptide corresponding to 16 amino acids near the amino-terminus of human BTK
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Western blot analysis of BTK in U937 cell lysate with BTK antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.
Sample Data :Immunocytochemistry of BTK in Daudi cells with BTK antibody at 10 ug/ml.
Scientific Background :BTK (also known as Bruton tyrosine kinase) plays a crucial role in B-lymphocyte differentiation and activation. BTK interacts with SRC homology 3 domains of FYN, LYN and HCK that are activated upon stimulation of B- and T-cell receptors (1). Defects in the BTK gene cause Agammaglobulinemia, an X-linked immunodeficiency characterized by failure to produce mature B lymphocyte cells and associated with a failure of Ig heavy chain rearrangement. The unique role of BTK makes it a desirable target for potential anti-cancer, anti-inflammatory and anti-viral agents as well as other treatments (2).
1. Cheng, G. et al: Binding of Bruton's tyrosine kinase to Fyn, Lyn, or Hck through a Src homology 3 domain-mediated interaction. Proc. Nat. Acad. Sci. 91: 8152-8155, 1994.
2. Vassilev, A O. et al: Therapeutic potential of inhibiting Bruton's tyrosine kinase, (BTK). Curr Pharm Des. 2004;10(15):1757-66.
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Research Areas :Cancer, Cytoplasmic Tyrosine Kinases, Inflammation, NfkB Pathway, Cancer, Inflammation, NfkB Pathway, Cytoplasmic Tyrosine Kinases