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Description :Mouse Monoclonal Antibody
Specificity :Recognizes the β-actin protein
Cited Applications :WB
Cross Reactivity :Western blot B-actin from human and mouse cells
B-actin from other species may also be detectable.
Host / Isotype / Clone# :Mouse, IgG2b, BA3R
Immunogen :Slightly modified human β-cytoplasmic actin (2-15aa) peptide
Formulation :10mM PBS, pH 7.2
Purification :Affinity Chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Representative western blot with Anti-β-Actin (1:1000) using 3 μg, 10 μg, and 30 μg of 293 cell lysate.
Scientific Background :Actin, a ubiquitous protein in eukaryotes, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controling cell structure and motility (1). α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate contractile potentials for muscle cells (1).
1. Herman, I.M. (1993) Curr. Opin. Cell Biol. 5, 48–55.
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Research Areas :Cancer, Invasion/Metastasis, Cancer, Invasion/Metastasis