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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the BAX protein
Cited Applications :WB, ELISA, ICC, IF
Cross Reactivity :Human
Host / Isotype / Clone# :Rabbit, IgG
Immunogen :BAX antibody was raised against a peptide corresponding to 16 amino acids near the amino-terminus of human BAX
Formulation :PBS + 0.02% sodium azide.
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Western blot analysis of BAX in HL-60 cell lysates with BAX antibody at (A) 1, (B) 2, and (C) 4 ug/ml.
Sample Data :Immunocytochemistry staining of HL-60 cells using BAX at 2 ug/ml.
Scientific Background :BAX is a proapoptotic protein of the BCL2 protein family. BAX forms a heterodimer with BCL2 and functions as an apoptotic activator. BAX interacts with and increases the opening of the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in the mitochondrial membrane potential and the release of cytochrome c (1). The expression of BAX gene is regulated by the tumor suppressor p53 and BAX has been shown to be involved in p53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene (2).
1. Deveraux Q L, et al: X-linked X-linked IAP is a direct inhibitor of cell-death proteases. Nature 388: 300-304, 1997.
2. Tang G, et al: Inhibition of JNK activation through NF- kappa-B target genes. Nature 414: 313-317, 2001.
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Research Areas :AKT/PKB Pathway, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Neurobiology, Cancer, Neurobiology, Cardiovascular Disease, AKT/PKB Pathway, Apoptosis/Autophagy