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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the ATF2 protein
Cited Applications :WB
Cross Reactivity :Western blot human and mouse ATF2
ATF2 from other species may also be detectable.
Immunogen :Synthetic peptide corresponding to amino acids 60-64 of human ATF2
Formulation :PBS pH 7.4, 0.02% NaN3, 50% glycerol
Purification :Affinity Chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Representative Western blot with Anti-ATF2 (1:1000) using 10ng of GST-tagged human recombinant ATF2.
Sample Data :Representative Western blot with Anti-ATF2 (1:1000) using 30 μg of HeLa, 293, Jurkat, THP1 and 3T6 cell lysates.
Scientific Background :Activating transcription factor (ATF)-2, also designated CRE-BP1, is a member of the ATF/cyclic AMP-responsive element binding protein family of transcription factors. ATF2 has two unique characteristics: it mediates the adenovirus E1A-induced trans-activation and forms a heterodimer with c-Jun (1). ATF2 is a target of the JNK signal transduction pathway (2). ATF2 is phosphorylated by JNK on two closely spaced threonine residues within the NH2-terminal activation domain. The replacement of these phosphorylation sites with alanine inhibited the transcriptional activity of ATF2. Furthermore, p38 MAPK can also phosphorylate ATF2.
1.Nomura, N. et al: Isolation and characterization of a novel member of the gene family encoding the cAMP response element-binding protein CRE-BP1.J Biol Chem. 1993 Feb 25; 268(6):4259-66.
2.Gupta, S. et al: Transcription factor ATF2 regulation by the JNK signal transduction pathway. Science. 1995 Jan 20; 267(5196):389-93.
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Research Areas :p38 Pathway, NfkB Pathway, Neurobiology, Metabolic Disorder, Inflammation, Cellular Stress, Cardiovascular Disease, Apoptosis/Autophagy, Angiogenesis, Neurobiology, Inflammation, Metabolic Disorder, Cardiovascular Disease, p38 Pathway, NfkB Pathway, Apoptosis/Autophagy, Cellular Stress, Angiogenesis