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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the ASK1 protein
Cited Applications :WB, ELISA, ICC, IF
Cross Reactivity :Human
Host / Isotype / Clone# :Rabbit, IgG
Immunogen :ASK1 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human ASK1. This sequence is different from that of mouse by last two amino acids.
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Western blot analysis of ASK1 in SW1353 whole cell lysate with ASK1 antibody at 1:500 dilution.
Sample Data :Immunocytochemistry of ASK1 in A431 cells with ASK1 antibody at 10 ug/ml.
Scientific Background :ASK1, also known as MAPKKK5, activates MKK3, MKK4 (SEK1), and MKK6. Overexpression of ASK1 induces apoptotic cell death, and ASK1 is activated in cells treated with tumor necrosis factor-alpha (1). ASK1 interacts with members of the TRAF family and is activated by TRAF2 in the TNF-signaling pathway. After activation by TRAF2, ASK1 activates MKK4, which in turn activates JNK. Thus, ASK1 is a mediator of TRAF2-induced JNK activation (2).
1. Smith, C. Ichijo, H. et al: Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways. Science 275: 90-94, 1997.
2. Nishitoh, H. et al: ASK1 is essential for JNK/SAPK activation by TRAF2. Molec. Cell 2: 389-395, 1998.
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Research Areas :Cardiovascular Disease, ERK/MAPK Pathway, Ser/Thr Kinases, Cardiovascular Disease, ERK/MAPK Pathway, Apoptosis/Autophagy, Cellular Stress, Ser/Thr Kinases