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Description :Mouse Monoclonal Antibody
Specificity :Recognizes the ASK1 protein
Cited Applications :WB
Cross Reactivity :Western blot of human ASK1
ASK1 from other species may also be detectable.
Host / Clone# :Mouse, A23
Immunogen :Human recombinant 649-946 ASK1
Formulation :10mM PBS, pH 7.2
Purification :Affinity Chromatography
Stability :Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Representative western blot (1:1000) with Anti-ASK1 (A23) using 20 ng of GST-tagged human full-length recombinant ASK1.
Scientific Background :ASK1, also known as MAPKKK5, activates MKK3, MKK4 (SEK1), and MKK6. Overexpression of ASK1 induces apoptotic cell death, and ASK1 is activated in cells treated with tumor necrosis factor-alpha (1). ASK1 interacts with members of the TRAF family and is activated by TRAF2 in the TNF-signaling pathway. After activation by TRAF2, ASK1 activates MKK4, which in turn activates JNK. Thus, ASK1 is a mediator of TRAF2-induced JNK activation (2).
1. Ichijo, H. et al: Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways. Science 275: 90-94, 1997.
2. Nishitoh, H. et al: ASK1 is essential for JNK/SAPK activation by TRAF2. Molec. Cell 2: 389-395, 1998.
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Research Areas :Cardiovascular Disease, ERK/MAPK Pathway, Apoptosis/Autophagy, Cellular Stress, Ser/Thr Kinases, Cardiovascular Disease, ERK/MAPK Pathway, Apoptosis/Autophagy, Cellular Stress, Ser/Thr Kinases