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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the human Cdc27 protein
Cited Applications :ELISA, IF, WB
Ideal working dilutions for each application should be empirically determined by the investigator.
Cross Reactivity :Human
Host Isotype / Clone# :Rabbit, IgG
Immunogen :The antibody was produced against synthesized peptide corresponding to amino acids 422-430 of human Cdc27
Formulation :0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 + 0.01% (w/v) Sodium Azide
Purification :Immunoaffinity chromatography
Stability :1yr at –20oC from date of shipment
Sample Data :Western blot using Anti-APC3 (CDC27) antibody (1.0ug/ml) shows detection of a band ~90 kDa corresponding to human cdc27 (arrowhead) in ~ 35 ug of HeLa whole cell lysate.
Scientific Background :Cdc27 (a.k.a. cell division cycle protein 27, CDC27Hs and H-NUC) is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits. APC catalyzes the formation of cyclin B-ubiquitin conjugate that controls the ubiquitin-mediated proteolysis of B-type cyclins. Cdc27 contains a TPR (tetratricopeptide repeat) domain, which facilitates protein-protein interactions. This protein interacts with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, pointing to a role in mitotic timing.
1. Topper,L.M., et al. The dephosphorylated form of the anaphase-promoting complex protein Cdc27/Apc3 concentrates on kinetochores and chromosome arms in mitosis. Cell Cycle. 2002:1 (4); 282-292.
2. Wassmann,K., et al.: Mad2 transiently associates with an APC/p55Cdc complex during mitosis. Proc. Natl. Acad. Sci. U.S.A. 1988: 95 (19); 11193-11198.
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Research Areas :Cancer, Cell Cycle, Neurobiology, p38 Pathway