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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the AOS1 protein
Cited Applications :WB
Cross Reactivity :Western blot AOS1 from human and mouse cells
AOS1 from other species may also be detectable.
Immunogen :Synthetic peptide of c-terminal amino acid sequence of human AOS1 (Q9UBE0)
Formulation :TBS, 50% glycerol
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Representative western blot with Anti-AOS1 (1:5000) using 3 μg, 10 μg and 30 μg of HeLa cell lysate.
Scientific Background :AOS1 encodes a member of the SUMO-activating enzyme for the sumoylation of proteins that regulates protein structure and intracellular localization. AOS1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins. Sumoylation is a 2-step reaction in which the SUA1/UBA2 dimer functions as an E1 enzyme and UBC9 functions as an E2 enzyme (1). AOS1 is required for sumoylation of UBA2 by epitope-tagged sentrin-1, sentrin-2 and sentrin-3 (2).
1. Okuma. et.al: In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2. Biochem. Biophys. Res. Commun. 254: 693-698, 1999.
2. Gong, L. et.al: Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex. FEBS Lett. 448: 185-189, 1999.
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Research Areas :Cancer, Neurobiology, Cancer, Neurobiology