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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the AKT3 protein
Cited Applications :WB
Cross Reactivity :Western blot AKT3 from human and mouse cells
AKT3 from other species may also be detectable.
Immunogen :Protein Code: Q9Y243
Formulation :TBS, 50% glycerol
Purification :Affinity Chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Representative western blot (1:1000) with Anti-AKT3 using 20 ng of human full length recombinant AKT1, AKT2, and AKT3.
Scientific Background :AKT 3 or Protein Kinase B (PKB ) is a serine/threonine kinase that is a member of the AKTγ (PKBγB family. AKT 3 is activated in cells exposed to diverse stimuli such as hormones, growth factors, and extracellular matrix components (1). AKT 3 phosphorylates and regulates the function of many cellular proteins involved in processes that include cellular metabolism, survival/apoptosis, and proliferation. Recent evidence indicates that AKT 3 is frequently overexpressed in many types of human cancers including breast and prostate (2).
1. Coffer, PJ. et al: Protein kinase B (c-Akt): a multifunctional mediator of phosphatidylinositol 3-kinase activation. Biochem J. 1998 Oct 1; 335 (Pt 1): 1-13.
2. Anderson, KE. et al: Translocation of PDK-1 to the plasma membrane is important in allowing PDK-1 to activate protein kinase B. Curr Biol. 1998 Jun 4;8(12): 684-91.
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Research Areas :Cancer, Neurobiology, AKT/PKB Pathway, NfkB Pathway, Apoptosis/Autophagy, Cell Cycle, Ser/Thr Kinases, Cancer, Neurobiology, Inflammation, Cell Cycle