Anti-AKT3(A18-61M)

Anti-AKT3(A18-61M)

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Description :Mouse Monoclonal Antibody

Species :

Tag :

Expression System:

Sequence :

Specificity  :Recognizes phosphorylated and unphosphorylated human AKT3 protein.

Cited Applications :ELISA, IHC, WB

Cross Reactivity :Human

Host Isotype / Clone# :Mouse, IgG1

Immunogen :The antibody was produced against synthesized peptide corresponding to internal residues of the human AKT3 protein.

Formulation :0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 + 0.01% (w/v) Sodium Azide

Purification :Protein A chromatography

Stability :1yr at –20oC from date of shipment

Sample Data :Western Blot of Anti-AKT3 antibody (1:1,000). Lane 1: C2C12. Lane 2: MEF#1. Lane 3: MEF#2. Lane 4: A549. Lane 5: Calu-1. Lane 6: PC3. Lane 7: HepG2. Lane 8: Jurkat. Lane 9: SKOV3. Lane 10: 293T. Load: 35 µg per lane.

Sample Data :Immunohistochemistry of Mouse Monoclonal anti AKT3 Antibody in Mouse Embryonic Kidney.

Scientific Background :AKT 3 or Protein Kinase B γ (PKBγ) is a serine/threonine kinase that is a member of the AKT family. AKT 3 is activated in cells exposed to diverse stimuli such as hormones, growth factors, and extracellular matrix components (1). AKT 3 phosphorylates and regulates the function of many cellular proteins involved in processes that include cellular metabolism, survival/apoptosis, and proliferation. Recent evidence indicates that AKT 3 is frequently overexpressed in many types of human cancers including breast and prostate (2).

References :
1. Coffer, PJ. et al: Protein kinase B (c-Akt): a multifunctional mediator of phosphatidylinositol 3-kinase activation. Biochem J. 1998 Oct 1; 335 (Pt 1): 1-13. 

2. Anderson, KE. et al: Translocation of PDK-1 to the plasma membrane is important in allowing PDK-1 to activate protein kinase B. Curr Biol. 1998 Jun 4;8(12): 684-91.

Product Sheets (By Lot #) :

B3216-15.pdf

Research Areas :AKT/PKB Pathway, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Inflammation, Invasion/Metastasis, Metabolic Disorder, Neurobiology, NfkB Pathway, WNT Signaling