Anti-AKT2(A17-61A)

Anti-AKT2(A17-61A)

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Description :Rat Monoclonal Antibody

Species :

Tag :

Expression System:

Sequence :

Specificity :Recognizes phosphorylated and unphosphorylated human AKT2 protein.

Cited Applications :ELISA, IHC, WB

Cross Reactivity :Human

Host Isotype / Clone# :Rat, IgG2a

Immunogen :The antibody was produced against synthesized peptide corresponding to internal residues of the human AKT2 protein.

Purification :Protein G chromatography

Stability :1yr at –20oC from date of shipment

Sample Data :Western Blot of Anti-AKT2 (1:1,000). Lane 1: C2C12. Lane 2: MEF#1. Lane 3: MEF#2. Lane 4: A549. Lane 5: Calu-1. Lane 6: PC3. Lane 7: HepG2. Lane 8: Jurkat. Lane 9: SKOV3. Lane 10: 293T. Load: 35 µg per lane.

Sample Data :Immunohistochemical analysis of FFPE buffered formalin 10% Conc. Fixed human breast carcinoma tissue using Anti-AKT2

Scientific Background :AKT2 or Protein Kinase B β (PKBβ) is a serine/threonine kinase that is a member of the AKT family. AKT2 like the other AKT members is activated in cells in response to diverse stimuli such as hormones, growth factors and extracellular matrix components and is involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, and cell motility. The PI3K generates phosphatidylinositol-3,4,5-trisphosphate (PIP3), a lipid second messenger essential for the translocation of AKT2 to the plasma membrane where it is phosphorylated and activated by phosphoinositide-dependent kinase-1 (PDK-1).

References :
1. Coffer, PJ. et al: Protein kinase B (c-Akt): a multifunctional mediator of phosphatidylinositol 3-kinase activation. Biochem J. 1998 Oct 1; 369 ( Pt 1):1-13. 

2. Anderson, KE. et al: Translocation of PDK-1 to the plasma membrane is important in allowing PDK-1 to activate protein kinase B. Curr Biol. 1998 Jun 4;8(12): 684-91. 

Product Sheets (By Lot #) :

B3216-14.pdf

Research Areas :AKT/PKB Pathway, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Inflammation, Invasion/Metastasis, Metabolic Disorder, Neurobiology, NfkB Pathway, WNT Signaling