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Description :Mouse Monoclonal Antibody
Specificity :Anti-AKT1 antibody recognizes the AKT protein.
Cited Applications :WB
Cross Reactivity :Western blot AKT1 from human and mouse cells
AKT from other species may also be detectable.
Host / Isotype / Clone# :Mouse, Monoclonal, 9A4
Immunogen :Recombinant full length active AKT1 protein
Formulation :0.1M TRIS pH 8.0, 0.1M glycine
Purification :Affinity purified using Protein G
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Representative western blot (1:2500) using 10µg, 20µg, and 40µg of HEK-293 cell lysate.
Scientific Background :AKT1/PKBα is a serine/threonine kinase that belongs to the AKT family. AKT1 is activated in cells in response to diverse stimuli such as hormones, growth factors and extracellular matrix components and is involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, and cell motility (1). AKT1 is frequently overexpressed and active in many types of human cancers including cancers of colon, breast, brain, pancreas and prostate as well as lymphomas and leukemias (2).
1. Coffer, PJ. et al: Protein kinase B (c-Akt): a multifunctional mediator of phosphatidylinositol 3-kinase activation. Biochem J. 1998 Oct 1; 335 (Pt 1): 1-13.
2. Anderson, KE. et al: Translocation of PDK-1 to the plasma membrane is important in allowing PDK-1 to activate protein kinase B. Curr Biol. 1998 Jun 4;8(12): 684-91.
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Research Areas :Cancer, Neurobiology, Inflammation, Metabolic Disorder, Cardiovascular Disease, AKT/PKB Pathway, NfkB Pathway, WNT Signaling, Apoptosis/Autophagy, Angiogenesis, Invasion/Metastasis, Cancer, Neurobiology, Inflammation, Cell Cycle