
Anti-AKT (pan)(A16-63R)
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Description :Rabbit Polyclonal Antibody
Species :
Tag :
Expression System:
Sequence :
Specificity :Recognizes the AKT1, AKT2, and AKT3 protein
Cited Applications :WB
Cross Reactivity :Western blot AKT1, AKT2, and AKT3 from human and mouse cells
AKT1, AKT2, and AKT3 from other species may also be detectable.
Host :Rabbit
Immunogen :Synthetic peptide corresponding to amino acids 471-475 of human AKT
Purification :Affinity chromatography
Stability :Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Representative western blot with Anti-AKT (pan) (1:1000) using 20 ng of human recombinant AKT1, AKT2, and AKT3.
Sample Data :Representative western blot with Anti-AKT (pan) (1:1000) using 30 μg of HeLa, HEK-293T, Jurkat, THP1, and 3T6 cell lysate.
Scientific Background :Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473 (1).
References :
1. Anderson, KE. et al: Translocation of PDK-1 to the plasma membrane is important in allowing PDK-1 to activate protein kinase B. Curr Biol. 1998 Jun 4;8(12): 684-91.
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Research Areas :Cancer, Neurobiology, Inflammation, Cell Cycle, Cancer, Neurobiology, Inflammation, Cell Cycle