Anti-AKT (pan)(A16-63R)

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Description :Rabbit Polyclonal Antibody

Species :

Tag :

Expression System:

Sequence :

Specificity :Recognizes the AKT1, AKT2, and AKT3 protein

Cited Applications :WB

Cross Reactivity :Western blot AKT1, AKT2, and AKT3 from human and mouse cells

AKT1, AKT2, and AKT3 from other species may also be detectable.

Host :Rabbit

Immunogen :Synthetic peptide corresponding to amino acids 471-475 of human AKT

Formulation :PBS pH 7.4, 0.02% sodium azide, 50% glycerol

Purification :Affinity chromatography

Stability :Store at 4°C (add 0.1% NaN₃) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data :Representative western blot with Anti-AKT (pan) (1:1000) using 20 ng of human recombinant AKT1, AKT2, and AKT3.

Sample Data :Representative western blot with Anti-AKT (pan) (1:1000) using 30 μg of HeLa, HEK-293T, Jurkat, THP1, and 3T6 cell lysate.

Scientific Background :Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473 (1).

References :
1. Anderson, KE. et al: Translocation of PDK-1 to the plasma membrane is important in allowing PDK-1 to activate protein kinase B. Curr Biol. 1998 Jun 4;8(12): 684-91.

Product Sheets (By Lot #) :

H270-4.pdf

T871-5.pdf

Research Areas :Cancer, Neurobiology, Inflammation, Cell Cycle, Cancer, Neurobiology, Inflammation, Cell Cycle