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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the AKT1, AKT2, and AKT3 protein
Cited Applications :WB
Cross Reactivity :Western blot AKT1, AKT2, and AKT3 from human and mouse cells
AKT1, AKT2, and AKT3 from other species may also be detectable.
Immunogen :Synthetic peptide corresponding to amino acids 471-475 of human AKT
Formulation :PBS pH 7.4, 0.02% sodium azide, 50% glycerol
Purification :Affinity chromatography
Stability :Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Representative western blot with Anti-AKT (pan) (1:1000) using 20 ng of human recombinant AKT1, AKT2, and AKT3.
Sample Data :Representative western blot with Anti-AKT (pan) (1:1000) using 30 μg of HeLa, HEK-293T, Jurkat, THP1, and 3T6 cell lysate.
Scientific Background :Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473 (1).
1. Anderson, KE. et al: Translocation of PDK-1 to the plasma membrane is important in allowing PDK-1 to activate protein kinase B. Curr Biol. 1998 Jun 4;8(12): 684-91.
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Research Areas :Cancer, Neurobiology, Inflammation, Cell Cycle, Cancer, Neurobiology, Inflammation, Cell Cycle