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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the AFX protein
Cited Applications :WB
Cross Reactivity :Western blot human and mouse AFX
AFX from other species may also be detectable.
Immunogen :Synthetic peptide corresponding to amino acids 195-199 of human AFX
Formulation :PBS pH 7.4, 0.02% NaN3, 50% glycerol
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Representative Western blot with Anti-AFX (1:500) using 3 μg, 10 μg and 30 μg of 3T6 cell lysate.
Scientific Background :AFX is a member of the O class of winged helix/forkhead transcription factor family. AFX proteins are involved in cell cycle regulation and inactivation of these proteins help in oncogenic transformation. AFX regulates apoptosis in part by suppressing the level of antiapoptotic BCLX through the transcriptional repressor BCL6 (1). AFX is down-regulated in patients with inflammatory bowel disease. AFX is expressed in human luteinized mural granulosa cells and may be involved in human folliculogenesis and luteinization. AFX acts as a novel anticancer agent for HER2-overexpressing cells.
1. Tang, T. et al: The forkhead transcription factor AFX activates apoptosis by induction of the BCL-6 transcriptional repressor. J. Biol. Chem. 27714255-14265, 2002.
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Research Areas :AKT/PKB Pathway, Apoptosis/Autophagy, Cancer, Cellular Stress, Inflammation, Neurobiology, Cancer, Neurobiology, Inflammation, AKT/PKB Pathway, Apoptosis/Autophagy, Cellular Stress