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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes acetylated lysine residues in proteins and peptide, including acetylated histone, acetylated BSA and acetylated MBP.
Cited Applications :WB, ELISA, PI, IHC, IF
Cross Reactivity :Pan-specific antibody. No reaction to non-acetylated proteins and peptides.
Immunogen :Acetylated lysine-KLH conjugates
Formulation :PBS, 50% glycerol, pH7
Purification :By affinity chromatography on an acetyl lysine agarose column
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Representative western blot of the acetylated protein profile with Anti-Acetylated Lysine (1:1000) using Hela cell lysate (A) and Hela cell lysate plus additional acetylated BSA (10 ng/μl) in the primary antibody reaction (B).
Scientific Background :Post-translational modification of proteins and peptides is a robust way to regulate function of existing proteins or peptides. Acetylation on lysine residues is one example of post-translational modification and is performed by a variety of acetyl transferases in the cell. Histone acetyl-transferases (HATs) are universal regulators of eukaryotic transcriptional activity and emerging therapeutic targets for human diseases (1). The Acetylated Lysine antibody detects Acetylation on lysine residues of proteins and peptide.
1. Mischerikow, N. et al: Targeted large-scale analysis of protein acetylation. Proteomics. 2011 Feb;11(4):571-89.
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Research Areas :Apoptosis/Autophagy, Cancer, Cell Cycle, Cancer, Apoptosis/Autophagy, Cell Cycle