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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the 4EBP1protein
Cited Applications :WB, ELISA, ICC
Cross Reactivity :Human and Mouse
Host / Isotype / Clone# :Rabbit, IgG
Immunogen :4EBP1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human 4EBP1
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Western blot analysis of 4EBP1 in 3T3 cell lysate with 4EBP1 antibody at (A) 2.5, (B) 5 and (C) 10 ug/ml.
Sample Data :Immunocytochemistry of 4EBP1in 3T3 cells with 4EBP1 antibody at 2 ug/ml.
Scientific Background :4EBP1 is a member of a family of translation repressor proteins that directly interact with eukaryotic translation initiation factor 4E (EIF4E). Interaction of 4EBP1 with EIF4E inhibits the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs thereby leading to repression of translation. Insulin treatment of adipose cells increases the phosphorylation of 4EBP1 and leads to reduced interaction of 4EBP1 with EIF4E (1). 4EBP1 is expressed in most tissues, with highest levels seen in adipose tissue, pancreas, and skeletal muscle (2).
1. Pause, A. et al: Insulin-dependent stimulation of protein synthesis by phosphorylation of a regulator of 5-prime-cap function. Nature 371: 762-767, 1994.
2. Tsukiyama-Kohara, K. et al: Tissue distribution, genomic structure, and chromosome mapping of mouse and human eukaryotic initiation factor 4E-binding proteins 1 and 2. Genomics 38: 353-363, 1996.
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Research Areas :AKT/PKB Pathway, Angiogenesis, Cancer, Metabolic Disorder, Cancer, Metabolic Disorder, AKT/PKB Pathway, Angiogenesis