Annexin 5 Protein(A61-30H)
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Description :Recombinant full-length human Annexin 5 Protein was expressed by E. coli cells using an N-terminal His tag.
Tag :HIS tag
Sequence :Full Length
Genbank Number :NM_001154
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Western Blot
Molecular Weight :~37 kDa
Gene Aliases :ANXA5, PP4, ANX5, ENX2
Scientific Background :Annexin 5 is a member of the annexin family of calcium-dependent phospholipid binding proteins. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. In vitro studies have shown that Annexin 5 is a cellular protein which plays a role in the inhibition of blood coagulation by competing for phosphatidylserine binding sites with prothrombin (1). Through affinity assays Annexin 5 has been localized on cells that expressed phosphatidylserine on the cell surface, a feature found in apoptosis as well as other forms of cell death (2).
1. Koopman, G. et al: Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis. Blood,1994; 84 (5): 1415–20.
2. Vermes, I. et al: A novel assay for apoptosis—flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V. J Immunol Methods,1995; 184 (1): 39
Formulation :50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 2mM DTT, 25% glycerol.
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Research Areas :Cancer, Neurobiology, Apoptosis/Autophagy, Cancer, Neurobiology, Apoptosis/Autophagy