AMSH, Active (A565-380H)
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Description : Recombinant full-length human AMSH was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.
Species : Human
Tag : His tag
Expression System : Sf9 insect cells using baculovirus
Sequence : Full Length
Genbank Number : NM_006463
Specific Activity : Sample Enzyme Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity : Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping : Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications : Activity Assay, Western Blot
Molecular Weight : 54 kDa
Gene Aliases : STAMBP, MICCAP
Scientific Background : AMSH (STAM binding protein) is a member of the JAMM metallo-enzyme family of deubiquitinating enzymes and is a ubiquitin isopeptidase found in the endosomes. It is involved in EGFR downregulation and regulates sorting of cell-surface receptors to the lysosome. The enzyme binds to the SH3 domain of STAM, which is required for MYC and cell growth (1). It has been hypothesized that mutations in AMSH are associated with Microcephaly-capillary malformation (2).
1. McCullough, John et al: AMSH is an endosome-associated ubiquitin isopeptidase. Journal of cell biology. vol. 166,4 (2004): 487-92.
2. McDonell, Laura M et al: Mutations in STAMBP, encoding a deubiquitinating enzyme, cause microcephaly-capillary malformation syndrome. Nature genetics 45(5): 556-62, 2013.
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Research Areas : Cancer, Cardiovascular Disease, Cell Cycle, Cellular Stress, Invasion/Metastasis, Metabolic Disorder