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Description :Recombinant full-length human AMPKG3 subunit was expressed by baculovirus in Sf9 insect cells using the N-terminal GST and C-terminal His tags.
Expression System:Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number :NM_017431
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Western Blot
Molecular Weight :~96 -108 kDa
Gene Aliases :PRKAG3
Formulation :50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Scientific Background :5'-AMP-activated protein kinase subunit gamma-3 (AMPKG3) is an AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. Ablation of the gamma3 subunit is associated with impaired glycogen resynthesis in skeletal muscle In contrast, naturally occurring mutations in the gamma3 subunit increase glycogen storage in skeletal muscle, which may enhance glycogen storage by shifting the metabolic fate of glucose from oxidation to storage.
1. Barnes BR, et al. 5'-AMP-activated protein kinase regulates skeletal muscle glycogen content and ergogenics. FASEB J. 19:773-9, 2005.
2. Mu J, et al. A role for AMP-activated protein kinase in contraction- and hypoxia-regulated glucose transport in skeletal muscle. Mol Cell. 7:1085-94, 2001.
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Research Areas :Cancer, Apoptosis/Autophagy, Ser/Thr Kinases, Metabolic Disorder, Cellular Stress