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Description :Recombinant full-length human AMPKG1 subunit was expressed by baculovirus in Sf9 insect cells using the N-terminal GST.
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number :NM_002733
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Western Blot
Molecular Weight :~68 kDa
Gene Aliases :PRKAG1, AMPKG, MGC8666
Formulation :50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Scientific Background :5'-AMP-activated protein kinase subunit gamma-3 (AMPKG1) is an AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. Ablation of the gamma-3 subunit is associated with impaired glycogen resynthesis in skeletal muscle In contrast, naturally occurring mutations in the gamma-3 subunit increase glycogen storage in skeletal muscle, which may enhance glycogen storage by shifting the metabolic fate of glucose from oxidation to storage.
1. Barnes BR, et al. 5'-AMP-activated protein kinase regulates skeletal muscle glycogen content and ergogenics. FASEB J. 19:773-9, 2005.
2. Mu J, et al. A role for AMP-activated protein kinase in contraction- and hypoxia-regulated glucose transport in skeletal muscle. Mol Cell. 7:1085-94, 2001.
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Research Areas :Apoptosis/Autophagy, Cancer, Cellular Stress, Metabolic Disorder, Ser/Thr Kinases