AMPK B1 siRNA Set I(P49-911)
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Description :AMPK is a pool of three individual synthetic siRNA duplexes designed to knock-down human AMPK mRNA expression. Each siRNA is 19-25 bases in length.
Specificity :AMPK siRNAs are designed to specifically knock-down human AMPK expression.
Formulation :The siRNAs are supplied as a lyophilized powder and shipped at room temperature.
Reconstitution Protocol :Briefly centrifuge the tubes (maximum RCF 4,000g) to collect lyophilized siRNA at the bottom of the tube. Resuspend the siRNA in 50 ul of DEPC-treated water (supplied by researcher), which results in a 1x stock solution (10 uM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot 1x stock solutions for storage.
Storage and Stability :The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ≥2 uM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Format :Lyophilized powder
Gene Aliases :PRKAB1, AMPK, HAMPKb, MGC17785
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Research Areas :Cancer, Metabolic Disorder, Apoptosis/Autophagy, Cellular Stress, Ser/Thr Kinases