AMPK (A2/B2/G2), Active(P54-10H)
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Description :Recombinant full-length human AMPK (combination of A2/B2/G2 subunits) was expressed by baculovirus in Sf9 insect cells using C-terminal His tags.
Tag :HIS tags
Expression System:Sf9 insect cells using baculovirus
Sequence :Full Length
Genbank Number :NM_006252
Genbank Number 2 :NM_005399
Genbank Number 3 :NM_001040633
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~69kDa (A2), ~36kDa (B2), ~65kDa (G2)
Gene Aliases :Subunit A2: PRKAA2, AMPK, AMPK2, PRKAA Subunit B2: PRKAB2, MGC61468 Subunit G2: PRKAG2, AAKG, CMH6, WPWS, AAKG2, H91620p
Scientific Background :AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis (1). AMPK exists as a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits. Binding of AMP to the γ subunit allosterically activates the complex. AMPK is activated in response to stresses that deplete cellular ATP (low glucose, hypoxia and ischemia) (2) and via signaling pathways in response to adiponectin, leptin and CAMKKβ.
1. Hardie, G.D. The AMP-activated protein kinase pathway – new players upstream and downstream. J. Cell Sci. 2004;117: 5479–5487.
2. Kahn, B.B. et al. AMP-activated protein kinase: Ancient energy gauge provides clues to modern understanding of metabolism. Cell Metab; 2005: 1, 15–25.
Formulation :50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Product Sheets (By Lot #) :
Research Areas :Apoptosis/Autophagy, Cancer, Cellular Stress, Metabolic Disorder, Ser/Thr Kinases, Cancer, Metabolic Disorder, Apoptosis/Autophagy, Cellular Stress, Ser/Thr Kinases