AMPK (A2/B2/G1), Active(P49M-10H)

AMPK (A2/B2/G1), Active(P49M-10H)

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Description :Recombinant full-length mouse AMPK (combination of A2/B2/G1 subunits) was expressed by baculovirus in Sf9 insect cells using a N-terminal His tag. The gene accession numbers for the three subunits (A2/B2/G1) are NM_178143, NM_182997, and NM_016781.

Species :Mouse

Tag :His tag

Expression System:Sf9 insect cells using baculovirus

Sequence :Full-length

Genbank Number :NM_178143

Genbank Number 2 :NM_182997

Genbank Number 3 :NM_016781

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay, Western Blot

Molecular Weight :68~72kDa (A2), ~35kDa (B2), and ~40kDa (G1)

Gene Aliases :Subunit A2: PRKAA2, A830082D05; AMPKalpha2
Subunit B2: PRKAB2, 5730553K21Rik; AW049591; BB124140
Subunit G1: PRKAG1, AA571379; BB036179; Prkaac

Formulation :50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.

Scientific Background :AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis (1). AMPK exists as a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits. Binding of AMP to the γ subunit allosterically activates the complex. AMPK is activated in response to stresses that deplete cellular ATP (low glucose, hypoxia and ischemia) (2) and via signaling pathways in response to adiponectin, leptin and CAMKKβ.

References :
1. Hardie, G.D. The AMP-activated protein kinase pathway – new players upstream and downstream. J. Cell Sci. 2004;117: 5479–5487.

2. Kahn, B.B. et al. AMP-activated protein kinase: Ancient energy gauge provides clues to modern understanding of metabolism. Cell Metab; 2005: 1, 15–25.

Product Sheets (By Lot #) :

V2430-4.pdf

Research Areas :Apoptosis/Autophagy, Cancer, Cellular Stress, Metabolic Disorder, Ser/Thr Kinases