AMPK (A2/B1/G3), Active(P85-10GH)

AMPK (A2/B1/G3), Active(P85-10GH)

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Description :Recombinant full-length human AMPK (combination of A2/B1/G3 subunits) was expressed by baculovirus in Sf9 insect cells using the N-terminal GST and C-terminal His tags.

Species :Human

Tag :GST/HIS

Expression System:Sf9 insect cells using baculovirus

Sequence :Full Length

Genbank Number :NM_006252

Genbank Number 2 :NM_006253

Genbank Number 3 :NM_017431

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay, Western Blot

Molecular Weight :~92 kDa (A2), ~65 kDa (B1), and ~108 kDa (G3)

Gene Aliases :Subunit A2: PRKAA2, AMPK, AMPK2, PRKAA Subunit B1: PRKAB1, AMPK, HAMPKb, MGC17785 Subunit G3: PRKAG3

Formulation :50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Scientific Background :AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis (1). AMPK exists as a heterotrimeric complex composed of a catalytic α subunit and regulatory beta and gamma subunits. Binding of AMP to the gamma subunit allosterically activates the complex. AMPK is activated in response to stresses that deplete cellular ATP (low glucose, hypoxia and ischemia) (2) and via signaling pathways in response to adiponectin, leptin and CAMKKbeta.

References :
1. Hardie, G.D. The AMP-activated protein kinase pathway – new players upstream and downstream. J. Cell Sci. 2004;117: 5479–5487.

2. Kahn, B.B. et al. AMP-activated protein kinase: Ancient energy gauge provides clues to modern understanding of metabolism. Cell Metab; 2005: 1, 15–25.

Product Sheets (By Lot #) :

Y900-3.pdf

Research Areas :Apoptosis/Autophagy, Cancer, Cellular Stress, Metabolic Disorder, Ser/Thr Kinases, Cancer, Metabolic Disorder, Apoptosis/Autophagy, Cellular Stress, Ser/Thr Kinases