AMPK(A1/B2/G1), Active(P50M-10H)

AMPK(A1/B2/G1), Active(P50M-10H)

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Description :Recombinant full-length mouse AMPK (combination of A1/B2/G1 subunits) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.

Species :Mouse

Tag :His tag

Expression System:Sf9 insect cells using baculovirus

Sequence :Full length

Genbank Number :NM_001013367

Genbank Number 2 :NM_182997

Genbank Number 3 :NM_016781

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay, Western Blot

Molecular Weight :68~72kDa (A1), ~35kDa (B2), and ~40kDa (G1)

Gene Aliases :Subunit A1: Prkaa1; AMPKalpha1; C130083N04Rik
Subunit B2: Prkab2; 5730553K21Rik; AW049591; BB124140
Subunit G1: Prkag1; AA571379; BB036179; Prkaac

Formulation :50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol

Scientific Background :AMPK (A1/B2/G1) is a member of the AMPK family which are heterotrimeric proteins consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPKs are an important energy-sensing enzyme group in the cells that monitor energy status particularly in response to stress (1). AMPKs regulate fatty acid and cholesterol synthesis by regulating the key rate-limiting enzymes acetyl-CoA carboxylase and hydroxy beta-methylglutaryl-CoA reductase. The β subunit may be a positive regulator of AMPK activity and is highly expressed in skeletal muscle (2).

References :
1. Viollet, B. et al: Physiological role of AMP-activated protein kinase (AMPK): insights from knockout mouse models. Biochem. Soc. Trans. 2003; 31; 216–219.

2. Thornton, C. et al: Identification of a novel AMP-activated protein kinase beta subunit isoform that is highly expressed in skeletal muscle. J. Biol. Chem. 273: 12443-12450, 1998.

Product Sheets (By Lot #) :

V2551-4.pdf

Research Areas :Cancer, Cellular Stress, Metabolic Disorder, Ser/Thr Kinases